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KMID : 0903520050480020115
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2005 Volume.48 No. 2 p.115 ~ p.119
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The Roles of Metal Ions and Water Molecules in the Hydrolysis of Bis(p-nitrophenyl)phosphate as a DNA Model Catalyzed by Dinuclear Ni(II) Complex
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Seong Nak-Do
Yun Ki-Seob
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Abstract
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The catalytic hydrolysis reactivities of dinuclear nickel (II) complex, μ-aquapentaaqua[μ-3,6-bis(6’- methyl-2’-pyridyl)pyridazine]chlorodinickel (II) trichloride trihydrate (APNT) for bis(p-nitrophenyl) phosphate (BNPP) as a DNA model compound were investigated. The dissociation constants of APNT were pKa1= 7.9 and pKa2= 9.6, respectively. The hydrolysis rate constant of BNPP compound by APNT was showed the rate enhancement of about 370,000 times in the case of none catalyst at pH 7.0 and 50oC. Based on the findings, we proposed the catalytic cycle for the hydrolysis of BNPP by APNT complex. The metal ions of dinuclear nickel (II) complex significantly enhance the transfer rate of phosphoryl group in the catalytic process and the water molecules as nucleophile and proton transfer agent act in different steps.
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KEYWORD
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Catalytic hydrolysis, bis(p-nitrophenyl)phosphate, dinuclear Ni (II) complex, water molecule, μ-aquapentaaqua [μ-3, 6-bis(6’-methyl-2’-pyridyl)pyridazine]chlorodinickel (II) trichloride trihydrate, metal ion
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